Raw Data for BglB Variant L122Q

Expression Data

SDS-PAGE
 0.68

Kinetic Assay Data

slope of L122Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.07530 0.2376 0.1256
B 25.00 0.1682 0.2138 0.1983
C 8.33 0.1633 0.1099 0.1450
D 2.78 0.1011 0.1032 0.1041
E 0.93 0.03910 0.04850 0.04550
F 0.31 0.01530 0.01990 0.01730
G 0.10 0.006200 0.005550
H 0.03 0.0003000 0.0003000
 0.68      10×

Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by ccabrera and last updated on 2024-12-20 21:12:54.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.39E-01 3.33E-01 3.31E-01
B 25.00 2.81E-01
C 8.33 2.81E-01 2.87E-01
D 2.78 2.01E-01 2.68E-01 1.93E-01
E 0.93 9.25E-02 9.43E-02 9.43E-02
F 0.31 3.19E-02 4.15E-02 3.19E-02
G 0.10 1.25E-02 1.27E-02 1.34E-02
H 0.03 0
 1.28      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by vgutta and last updated on 2024-12-20 18:30:29.


Thermostability Assay Data

slope of L122Q A420 nm (min−1)
T (°C) 1 2 3
A 30.0
B 48.3 0.0005000 0.0007000
C 45.7 0.0009000 0.0006000 0.0007000
D 42.4 0.001600 0.001900 0.002100
E 37.7 0.07660 0.07050 0.07080
F 33.6 0.1643 0.1643 0.1649
G 31.3 0.1982 0.1999 0.1795
H 50.0

Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by ccabrera and last updated on 2024-12-20 21:14:00.


Reference Data for WT

slope of L122Q A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 1.10E-03 1.10E-03
D 42.4 9.70E-02 8.64E-02 8.24E-02
E 37.7 4.62E-01 4.81E-01 3.93E-01
F 33.6 4.48E-01 4.32E-01 4.42E-01
G 31.3 4.58E-01 4.52E-01 4.39E-01
H 30.0 4.46E-01 4.49E-01 4.59E-01

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by vgutta and last updated on 2024-11-20 12:30:52.


Design Data

7.943

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.