Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.35

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.5247	0.5256	0.5216
	B	25.00	0.4892	0.4143	0.4612
	C	8.33	0.3967	0.3530	0.3534
	D	2.78	0.2508	0.2718	0.2323
	E	0.93	0.11700	0.11980	0.13790
	F	0.31	0.05700	0.05310
	G	0.10	0.022600	0.017800	0.029250
	H	0.03	0.001600	0.001400	0.001750

Yield (mg/mL): 1.35     Dilution Factor:  100×

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Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 11:44:07.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0007	0.0006	0.0007
	B	48.3	0.0009	0.0008	0.0010
	C	45.7	0.0009	0.0008	0.0008
	D	42.4	0.0550	0.0482	0.0703
	E	37.7	0.43300	0.39770	0.42040
	F	33.6	0.46650	0.42670	0.43730
	G	31.3	0.472900	0.503300	0.463300
	H	30.0	0.467600	0.495700	0.482000

Download these data

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 12:07:31.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.