Raw Data for BglB Variant N421D

Expression Data

SDS-PAGE
 1.12

Kinetic Assay Data

slope of N421D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.97E-01 3.09E-01 3.38E-01
B 25.00 2.54E-01 3.25E-01 3.40E-01
C 8.33 2.49E-01 2.63E-01 2.55E-01
D 2.78 2.13E-01 2.19E-01
E 0.93 7.59E-02 9.32E-02 8.09E-02
F 0.31 3.46E-02 3.28E-02
G 0.10 9.70E-03 1.16E-02 1.07E-02
H 0.03 0.00E+00 0.00E+00 0.00E+00
 1.12      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by MatthewEvers and last updated on 2024-11-20 12:02:05.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.39E-01 3.33E-01 3.31E-01
B 25.00 2.81E-01
C 8.33 2.81E-01 2.87E-01
D 2.78 2.01E-01 2.68E-01 1.93E-01
E 0.93 9.25E-02 9.43E-02 9.43E-02
F 0.31 3.19E-02 4.15E-02 3.19E-02
G 0.10 1.25E-02 1.27E-02 1.34E-02
H 0.03 0
 1.28      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by vgutta and last updated on 2024-12-20 18:30:29.


Thermostability Assay Data

slope of N421D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0007000 0.001000 0.001100
B 48.3 0.001200 0.001000 0.0009000
C 45.7 0.001300 0.001300 0.001200
D 42.4 0.1023 0.09480
E 37.7 0.3572 0.3433 0.3540
F 33.6 0.3682 0.3786 0.3703
G 31.3 0.3850 0.3976 0.3955
H 30.0 0.3845 0.3804 0.3810

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by MatthewEvers and last updated on 2024-11-20 12:18:49.


Reference Data for WT

slope of N421D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 1.10E-03 1.10E-03
D 42.4 9.70E-02 8.64E-02 8.24E-02
E 37.7 4.62E-01 4.81E-01 3.93E-01
F 33.6 4.48E-01 4.32E-01 4.42E-01
G 31.3 4.58E-01 4.52E-01 4.39E-01
H 30.0 4.46E-01 4.49E-01 4.59E-01

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by vgutta and last updated on 2024-11-20 12:30:52.


Design Data

-0.191

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.