Raw Data for BglB Variant N184D

Expression Data

SDS-PAGE
 1.31

Kinetic Assay Data

slope of N184D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.4173 0.4360 0.4376
B 25.00 0.3626 0.3686 0.3816
C 8.33 0.2550 0.2410 0.2542
D 2.78 0.1858 0.2105 0.2160
E 0.93 0.09620 0.09575 0.11060
F 0.31 0.04445 0.03655 0.03975
G 0.10 0.022300 0.016450 0.017800
H 0.03 0.001300 0.001300 0.001300
 1.31      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by QDNd2d and last updated on 2024-11-20 11:56:43.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.5247 0.5256 0.5216
B 25.00 0.4892 0.4143 0.4612
C 8.33 0.3967 0.3530 0.3534
D 2.78 0.2508 0.2718 0.2323
E 0.93 0.11700 0.11980 0.13790
F 0.31 0.05700 0.05310
G 0.10 0.022600 0.017800 0.029250
H 0.03 0.001600 0.001400 0.001750
 1.35      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 11:44:07.


Thermostability Assay Data

slope of N184D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0005000 0.0005000 0.0005000
B 48.3 0.0004000
C 45.7 0.001000 0.001100 0.001200
D 42.4 0.1306 0.07260 0.09280
E 37.7 0.3285 0.3897
F 33.6 0.4202 0.3849 0.4118
G 31.3 0.3632 0.4156 0.4126
H 30.0 0.4134 0.4288 0.3919

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by QDNd2d and last updated on 2024-11-20 12:22:11.


Reference Data for WT

slope of N184D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0007 0.0006 0.0007
B 48.3 0.0009 0.0008 0.0010
C 45.7 0.0009 0.0008 0.0008
D 42.4 0.0550 0.0482 0.0703
E 37.7 0.43300 0.39770 0.42040
F 33.6 0.46650 0.42670 0.43730
G 31.3 0.472900 0.503300 0.463300
H 30.0 0.467600 0.495700 0.482000

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 12:07:31.


Design Data

-0.176

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.