Raw Data for BglB Variant G271D

Expression Data

SDS-PAGE
 0.25

Kinetic Assay Data

slope of G271D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.06675 0.07495
B 25.00 0.05230 0.06160 0.06540
C 8.33 0.04850 0.05355 0.06255
D 2.78 0.04155 0.03735 0.03275
E 0.93 0.02160 0.01680 0.01840
F 0.31 0.007750 0.006400 0.007300
G 0.10 0.002150 0.002350 0.002300
H 0.03 0.0003000 0.0002000 0
 0.25      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by VictoriaT and last updated on 2024-11-20 12:32:35.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.39E-01 3.33E-01 3.31E-01
B 25.00 2.81E-01
C 8.33 2.81E-01 2.87E-01
D 2.78 2.01E-01 2.68E-01 1.93E-01
E 0.93 9.25E-02 9.43E-02 9.43E-02
F 0.31 3.19E-02 4.15E-02 3.19E-02
G 0.10 1.25E-02 1.27E-02 1.34E-02
H 0.03 0
 1.28      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by vgutta and last updated on 2024-12-20 18:30:29.


Thermostability Assay Data

slope of G271D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002000 0.0009000 0
B 48.3 0.002000 0.001200 0.0008000
C 45.7 0.0008000 0.0009000 0.001100
D 42.4 0.001100 0.001200
E 37.7 0.01470 0.01630 0.01370
F 33.6 0.04790 0.04670 0.04510
G 31.3 0.06760 0.06730 0.06110
H 30.0 0.05770 0.05840 0.05720

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by VictoriaT and last updated on 2024-11-20 12:30:02.


Reference Data for WT

slope of G271D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 1.10E-03 1.10E-03
D 42.4 9.70E-02 8.64E-02 8.24E-02
E 37.7 4.62E-01 4.81E-01 3.93E-01
F 33.6 4.48E-01 4.32E-01 4.42E-01
G 31.3 4.58E-01 4.52E-01 4.39E-01
H 30.0 4.46E-01 4.49E-01 4.59E-01

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by vgutta and last updated on 2024-11-20 12:30:52.


Design Data

4.701

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.