Raw Data for BglB Variant N270G

Expression Data

SDS-PAGE
 1.31

Kinetic Assay Data

slope of N270G A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.6726 0.6379
B 25.00 0.3809 0.3502 0.3641
C 8.33 0.2771 0.2848 0.2743
D 2.78 0.1901 0.1897 0.1817
E 0.93 0.09510 0.09280 0.10230
F 0.31 0.04470 0.03800 0.03975
G 0.10 0.018500 0.016150 0.013800
H 0.03 0.001350 0.000800 0.001150
 1.31      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by OscarR and last updated on 2024-11-20 12:02:00.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.5247 0.5256 0.5216
B 25.00 0.4892 0.4143 0.4612
C 8.33 0.3967 0.3530 0.3534
D 2.78 0.2508 0.2718 0.2323
E 0.93 0.11700 0.11980 0.13790
F 0.31 0.05700 0.05310
G 0.10 0.022600 0.017800 0.029250
H 0.03 0.001600 0.001400 0.001750
 1.35      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 11:44:07.


Thermostability Assay Data

slope of N270G A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00080 0.0007000 0.000700
B 48.3 0.0008000 0.000800
C 45.7 0.001000 0.001300 0.001200
D 42.4 0.08440 0.10430 0.08200
E 37.7 0.3791 0.3792 0.3788
F 33.6 0.4156 0.4245 0.3961
G 31.3 0.4313 0.4165 0.4188
H 30.0 0.4380 0.4018 0.4403

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by OscarR and last updated on 2024-11-20 12:13:17.


Reference Data for WT

slope of N270G A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0007 0.0006 0.0007
B 48.3 0.0009 0.0008 0.0010
C 45.7 0.0009 0.0008 0.0008
D 42.4 0.0550 0.0482 0.0703
E 37.7 0.43300 0.39770 0.42040
F 33.6 0.46650 0.42670 0.43730
G 31.3 0.472900 0.503300 0.463300
H 30.0 0.467600 0.495700 0.482000

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 12:07:31.


Design Data

3.159

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.