Raw Data for BglB Variant C38S

Expression Data

SDS-PAGE
 1.3

Kinetic Assay Data

slope of C38S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.4976 0.5152 0.5291
B 25.00 0.3175 0.3429 0.3718
C 8.33 0.2297 0.2518 0.2396
D 2.78 0.1494 0.1491 0.1458
E 0.93 0.05460 0.05690 0.05855
F 0.31 0.01845 0.01965 0.02145
G 0.10 0.006150 0.006900 0.007500
H 0.03 0.003000 0.003050 0.003200
 3.3      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by daobispo and last updated on 2024-11-20 12:16:06.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3217 0.3208 0.3160
B 25.00 0.2632 0.2678 0.2543
C 8.33 0.2580 0.21740 0.1913
D 2.78 0.1417 0.151400 0.1573
E 0.93 0.06825 0.066150 0.06180
F 0.31 0.02710 0.02625 0.02360
G 0.10 0.009200 0.00885 0.007850
H 0.03 0.004150 0.0039 0.003650
 0.82      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:15:24.


Thermostability Assay Data

slope of C38S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0006000 0.001000
B 48.3
C 45.7 0.001500 0.001100 0.0006000
D 42.4 0.1422 0.1314 0.1079
E 37.7 0.3276 0.3054 0.3401
F 33.6 0.2595 0.2668
G 31.3 0.3152 0.3213 0.3345
H 30.0 0.3537 0.3474

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by daobispo and last updated on 2024-11-20 12:15:25.


Reference Data for WT

slope of C38S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00100 0.000700
B 48.3 0.0006000
C 45.7 0.000900 0.0009
D 42.4 0.04390 0.05580 0.0707
E 37.7 0.2862 0.3131 0.3533
F 33.6 0.2663 0.2985 0.2944
G 31.3 0.2921 0.2913 0.2726
H 30.0 0.3055 0.3273 0.3522

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:22:40.


Design Data

1.081

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.