Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.82

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3217	0.3208	0.3160
	B	25.00	0.2632	0.2678	0.2543
	C	8.33	0.2580	0.21740	0.1913
	D	2.78	0.1417	0.151400	0.1573
	E	0.93	0.06825	0.066150	0.06180
	F	0.31	0.02710	0.02625	0.02360
	G	0.10	0.009200	0.00885	0.007850
	H	0.03	0.004150	0.0039	0.003650

Yield (mg/mL): 0.82     Dilution Factor:  100×

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Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:15:24.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00100		0.000700
	B	48.3	0.0006000
	C	45.7		0.000900	0.0009
	D	42.4	0.04390	0.05580	0.0707
	E	37.7	0.2862	0.3131	0.3533
	F	33.6	0.2663	0.2985	0.2944
	G	31.3	0.2921	0.2913	0.2726
	H	30.0	0.3055	0.3273	0.3522

Download these data

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:22:40.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.