Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.59

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.28E-01	5.14E-01	5.20E-01
	B	25.00	4.31E-01	4.06E-01	4.27E-01
	C	8.33	3.86E-01	3.60E-01	3.65E-01
	D	2.78	2.23E-01	2.25E-01	2.29E-01
	E	0.93	1.04E-01	1.02E-01	1.05E-01
	F	0.31	3.98E-02	3.95E-02	4.15E-02
	G	0.10	1.39E-02	1.41E-02	1.30E-02
	H	0.03		7.00E-04	8.50E-04

Yield (mg/mL): 1.59     Dilution Factor:  100×

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Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:36:41.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.0012000	0.000600
	B	48.3	0.0011		0.0009000
	C	45.7	0.0011	0.001100	0.001200
	D	42.4	0.0791	0.09140	0.11770
	E	37.7	0.6272	0.5683	0.5244
	F	33.6	0.6226	0.6248	0.6226
	G	31.3	0.6319	0.6288	0.6255
	H	30.0	0.628	0.6750	0.6181

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Protein purified on 11/6/2024 and assayed on 11/6/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:53:49.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.