Raw Data for BglB Variant E250D

Expression Data

SDS-PAGE
 0.77

Kinetic Assay Data

slope of E250D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.71E-01 1.92E-01 2.04E-01
B 25.00 1.40E-01 1.52E-01 1.59E-01
C 8.33 1.18E-01 1.34E-01 1.22E-01
D 2.78 1.16E-01 1.18E-01 1.09E-01
E 0.93 8.21E-02 6.74E-02 6.15E-02
F 0.31 6.53E-02 3.94E-02 4.89E-02
G 0.10 1.97E-02 2.15E-02
H 0.03 8.55E-03 9.10E-03
 0.77      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jefferytsong and last updated on 2024-11-20 15:00:21.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.30E-01 3.42E-01 3.12E-01
B 25.00 2.46E-01 2.88E-01
C 8.33 1.89E-01 1.75E-01
D 2.78 1.75E-01 1.81E-01
E 0.93
F 0.31 7.41E-02 7.83E-02
G 0.10 3.19E-02 3.39E-02
H 0.03 1.41E-02 1.03E-02 1.74E-02
 0.65      100×

Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by Mehek and last updated on 2024-11-20 15:13:44.


Thermostability Assay Data

slope of E250D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 7.00E-04 0
B 48.3 0 0
C 45.7 0 0 0
D 42.4 2.93E-02 2.20E-02 3.31E-02
E 37.7 2.36E-01 2.37E-01
F 33.6 2.86E-01 2.67E-01 3.50E-01
G 31.3 2.52E-01 2.15E-01 2.44E-01
H 30.0 1.96E-01 2.89E-01 2.33E-01

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by jefferytsong and last updated on 2024-11-20 15:32:20.


Reference Data for WT

slope of E250D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.70E-03 2.20E-03 2.80E-03
B 48.3 9.00E-04 1.10E-03
C 45.7 0 0
D 42.4 4.38E-02 3.54E-02 3.15E-02
E 37.7 3.62E-01 3.41E-01 3.30E-01
F 33.6 3.34E-01 3.39E-01 3.85E-01
G 31.3 3.90E-01 3.82E-01 4.13E-01
H 30.0 3.52E-01 4.08E-01 3.43E-01

Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by Mehek and last updated on 2024-11-20 15:31:52.


Design Data

-7.56

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.