Raw Data for BglB Variant T303K

Expression Data

SDS-PAGE
 1.17

Kinetic Assay Data

slope of T303K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2855 0.3162 0.3059
B 25.00 0.2545 0.2701 0.2200
C 8.33 0.1961 0.1979 0.2268
D 2.78 0.1364 0.1532 0.1155
E 0.93 0.06440 0.07531 0.06900
F 0.31 0.03320 0.03560 0.03545
G 0.10 0.013150 0.011800 0.012300
H 0.03 0 0 0
 1.17      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jazlyndias and last updated on 2024-11-20 14:53:32.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2862 0.2587 0.2615
B 25.00 0.2273 0.2437 0.2329
C 8.33 0.2244 0.2128 0.1853
D 2.78 0.1298 0.1306 0.1326
E 0.93 0.04780 0.05910
F 0.31 0.01795 0.02480 0.02665
G 0.10 0.008100 0.008050 0.009050
H 0.03 0.000250 0.000400 0
 1.21      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jazlyndias and last updated on 2024-11-20 15:02:47.


Thermostability Assay Data

slope of T303K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0006000 0.0007000
B 48.3 0.0005000 0.0007000 0.0006000
C 45.7 0.001300 0.001200 0.001300
D 42.4 0.1075 0.1045 0.1154
E 37.7 0.2485 0.2616 0.2371
F 33.6 0.2522 0.2477 0.2611
G 31.3 0.2476 0.2552 0.2484
H 30.0 0.2378 0.2611 0.2579

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by seaguo and last updated on 2024-11-20 15:32:23.


Reference Data for WT

slope of T303K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.000600 0.0015000 0
B 48.3 0.0005000 0.000500 0.0005000
C 45.7 0.001000 0.001100 0.001300
D 42.4 0.06780 0.06370 0.07000
E 37.7 0.2456 0.2398 0.2423
F 33.6 0.2568 0.2633 0.2403
G 31.3 0.2531 0.2537 0.2135
H 30.0 0.2613 0.2603 0.2774

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jazlyndias and last updated on 2024-11-20 15:14:11.


Design Data

76.029

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.