Raw Data for BglB Variant R414K

Expression Data

SDS-PAGE
 0.55

Kinetic Assay Data

slope of R414K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.5368 0.5162 0.4916
B 25.00 0.2217 0.2199 0.2329
C 8.33 0.1722 0.1808 0.1673
D 2.78 0.1513 0.1576 0.1667
E 0.93 0.1199 0.1115 0.1116
F 0.31 0.07370 0.07530 0.08470
G 0.10 0.03915 0.03830 0.04535
H 0.03 0.01565 0.01850 0.02145
 0.55      10×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by BhargavKapur and last updated on 2024-11-20 15:06:38.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.30E-01 3.42E-01 3.12E-01
B 25.00 2.46E-01 2.88E-01
C 8.33 1.89E-01 1.75E-01
D 2.78 1.75E-01 1.81E-01
E 0.93
F 0.31 7.41E-02 7.83E-02
G 0.10 3.19E-02 3.39E-02
H 0.03 1.41E-02 1.03E-02 1.74E-02
 0.65      100×

Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by Mehek and last updated on 2024-11-20 15:13:44.


Thermostability Assay Data

slope of R414K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002700 0.0006000 0.001300
B 48.3 0.0006000 0.0006000 0.0006000
C 45.7
D 42.4 0.02790 0.01980 0.03090
E 37.7 0.1555 0.1431 0.1525
F 33.6 0.1638 0.1121 0.1435
G 31.3 0.1320 0.1172 0.1597
H 30.0 0.1281 0.1347

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by BhargavKapur and last updated on 2024-11-20 15:22:04.


Reference Data for WT

slope of R414K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.70E-03 2.20E-03 2.80E-03
B 48.3 9.00E-04 1.10E-03
C 45.7 0 0
D 42.4 4.38E-02 3.54E-02 3.15E-02
E 37.7 3.62E-01 3.41E-01 3.30E-01
F 33.6 3.34E-01 3.39E-01 3.85E-01
G 31.3 3.90E-01 3.82E-01 4.13E-01
H 30.0 3.52E-01 4.08E-01 3.43E-01

Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by Mehek and last updated on 2024-11-20 15:31:52.


Design Data

1.575

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.