D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F415H

Expression Data

Yield (mg/mL): 0.47

Kinetic Assay Data

**slope of F415H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	0.0014	0.0017	0.0015
C	8.33		0.0007	0.0007
D	2.78
E	0.93	0.00070	0.00060	0.00065
F	0.31	0.00040	0.00040	0.00045
G	0.10	0.000250	0.000350	0.000350
H	0.03	0.000250		0.000250

Yield (mg/mL): 0.47 Dilution Factor: 100×

Protein purified on 11/23/2024 and assayed on 11/30/2024.
Data uploaded by enqif and last updated on 2024-11-20 15:01:32.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.30E-01	3.42E-01	3.12E-01
B	25.00	2.46E-01	2.88E-01
C	8.33	1.89E-01	1.75E-01
D	2.78	1.75E-01	1.81E-01
E	0.93
F	0.31	7.41E-02	7.83E-02
G	0.10	3.19E-02	3.39E-02
H	0.03	1.41E-02	1.03E-02	1.74E-02

Yield (mg/mL): 0.65 Dilution Factor: 100×

Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by Mehek and last updated on 2024-11-20 15:13:44.

Thermostability Assay Data

Data missing or deleted from the database.

Design Data

Rosetta Score for F415H: 2.302

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.