Raw Data for BglB Variant E250D

Expression Data

SDS-PAGE
 1.01

Kinetic Assay Data

slope of E250D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2684 0.2784 0.2738
B 25.00 0.2205 0.2181 0.2104
C 8.33 0.1770 0.1767 0.1718
D 2.78 0.1148 0.1102 0.1084
E 0.93 0.05100 0.05880 0.06565
F 0.31 0.04360 0.05570
G 0.10 0.008050 0.006750
H 0.03 0.0002500 0.0003000
 1.01      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by LiamDavis and last updated on 2024-11-20 15:07:37.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2475 0.2664 0.2619
B 25.00 0.1969 0.2008 0.2013
C 8.33 0.1699 0.1889 0.1857
D 2.78 0.1108 0.1193 0.1176
E 0.93 0.07625 0.07150 0.06280
F 0.31 0.06425 0.05800 0.06540
G 0.10 0.009950 0.01315 0.009850
H 0.03 0.0002000 0.0001500 0
 0.91      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by LiamDavis and last updated on 2024-12-07 00:01:34.


Thermostability Assay Data

slope of E250D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 8.00E-04 8.00E-04
B 48.3 2.00E-03 1.90E-03 2.10E-03
C 45.7 5.60E-03 5.20E-03
D 42.4 9.01E-02 7.41E-02 8.11E-02
E 37.7 2.60E-01 2.47E-01 2.47E-01
F 33.6 2.75E-01 2.60E-01 2.60E-01
G 31.3 2.87E-01 2.78E-01 2.81E-01
H 30.0 2.92E-01 2.82E-01 2.79E-01

Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by LiamDavis and last updated on 2024-11-20 15:46:37.


Reference Data for WT

slope of E250D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.20E-03 1.00E-03
B 48.3 1.90E-03 2.10E-03 1.60E-03
C 45.7 7.50E-03 6.90E-03
D 42.4 6.96E-02 6.64E-02
E 37.7 2.23E-01 2.36E-01 2.32E-01
F 33.6 2.38E-01 2.36E-01 2.40E-01
G 31.3 2.58E-01 2.63E-01 2.61E-01
H 30.0 2.74E-01 2.64E-01 2.55E-01

Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by LiamDavis and last updated on 2024-11-20 15:31:19.


Design Data

5.151

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.