Raw Data for BglB Variant G271D

Expression Data

Yield (mg/mL): 0.53

Kinetic Assay Data

slope of G271D A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.009450	0.01165	0.005550
	B	25.00	0.01150	0.01100	0.01335
	C	8.33	0.01055	0.01300	0.01285
	D	2.78	0.009000	0.009250	0.008700
	E	0.93	0.008350	0.007600
	F	0.31	0.003400	0.003850	0.004600
	G	0.10	0.001950	0.001850	0.001650
	H	0.03	0	0

Yield (mg/mL): 0.53     Dilution Factor:  100×

Download these data

Protein purified on 10/23/24 and assayed on 11/20/24.
Data uploaded by CheungEach and last updated on 2024-11-20 15:02:34.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1935	0.1974	0.2006
	B	25.00	0.1839	0.1755	0.1746
	C	8.33	0.1570	0.1403	0.1394
	D	2.78	0.1276	0.1393	0.1317
	E	0.93	0.06295	0.06160	0.07165
	F	0.31	0.02480	0.02910	0.03175
	G	0.10	0.009800	0.009900	0.012500
	H	0.03	0.000200		0.000200

Yield (mg/mL): 1.07     Dilution Factor:  100×

Download these data

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by MuhammadK and last updated on 2024-11-20 15:24:44.

Thermostability Assay Data

slope of G271D A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0.0008000	0.0005000
	B	48.3	0.0007000	0.0006000	0.0006000
	C	45.7	0.0006000		0.001000
	D	42.4	0.001100	0.001000	0.001100
	E	37.7	0.005700	0.006000
	F	33.6	0.02070	0.01870	0.01720
	G	31.3	0.02440	0.02280	0.02440
	H	30.0	0.02290	0.02280

Download these data

Protein purified on and assayed on .
Data uploaded by CheungEach and last updated on 2024-11-20 15:20:20.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.