Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.95

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3800	0.3190	0.3732
	B	25.00	0.3007	0.3739	0.2900
	C	8.33	0.2780	0.2833	0.2466
	D	2.78	0.1580	0.1569	0.1614
	E	0.93	0.07455	0.07365	0.08170
	F	0.31	0.02520	0.02790	0.02520
	G	0.10	0.00965	0.008600	0.011250
	H	0.03	0		0

Yield (mg/mL): 0.95     Dilution Factor:  100×

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Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:20:19.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0.0030000	0.001100
	B	48.3	0.0010000	0.001000	0.0012000
	C	45.7	0.001400	0.001500	0.001300
	D	42.4	0.05120	0.05190	0.06370
	E	37.7	0.3834	0.3837	0.3719
	F	33.6	0.3993	0.3857	0.4034
	G	31.3	0.3932	0.4149	0.4068
	H	30.0	0.4266	0.4082	0.4074

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Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:12:50.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.