Raw Data for BglB Variant L269V

Expression Data

SDS-PAGE
 0.43

Thermostability Assay Data

slope of L269V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0007 0.0005 0
B 48.3 0.0006 0.0006
C 45.7 0.0008 0.0007
D 42.4 0.0418 0.0341 0.0329
E 37.7 0.1589 0.1546
F 33.6 0.1802 0.2161 0.2248
G 31.3 0.1868 0.1895 0.1965
H 30.0 0.1507 0.1421

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by MitaD and last updated on 2024-11-20 15:28:17.


Reference Data for WT

slope of L269V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.0030000 0.001100
B 48.3 0.0010000 0.001000 0.0012000
C 45.7 0.001400 0.001500 0.001300
D 42.4 0.05120 0.05190 0.06370
E 37.7 0.3834 0.3837 0.3719
F 33.6 0.3993 0.3857 0.4034
G 31.3 0.3932 0.4149 0.4068
H 30.0 0.4266 0.4082 0.4074

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:12:50.



Design Data

21.647


Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.