Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.91

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2475	0.2664	0.2619
	B	25.00	0.1969	0.2008	0.2013
	C	8.33	0.1699	0.1889	0.1857
	D	2.78	0.1108	0.1193	0.1176
	E	0.93	0.07625	0.07150	0.06280
	F	0.31	0.06425	0.05800	0.06540
	G	0.10	0.009950	0.01315	0.009850
	H	0.03	0.0002000	0.0001500	0

Yield (mg/mL): 0.91     Dilution Factor:  100×

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Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by LiamDavis and last updated on 2024-12-07 00:01:34.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.20E-03	1.00E-03
	B	48.3	1.90E-03	2.10E-03	1.60E-03
	C	45.7		7.50E-03	6.90E-03
	D	42.4		6.96E-02	6.64E-02
	E	37.7	2.23E-01	2.36E-01	2.32E-01
	F	33.6	2.38E-01	2.36E-01	2.40E-01
	G	31.3	2.58E-01	2.63E-01	2.61E-01
	H	30.0	2.74E-01	2.64E-01	2.55E-01

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Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by LiamDavis and last updated on 2024-11-20 15:31:19.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.