D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I327G

Yield (mg/mL): 0.112

**slope of I327G A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.00E+00	8.00E+00	9.00E+00
B	25.00	1.67E-01	1.71E-01	1.78E-01
C	8.33	7.90E-02	6.20E-02	8.60E-02
D	2.78	6.00E-02	5.20E-02	5.80E-02
E	0.93	4.90E-02	4.40E-02	4.80E-02
F	0.31	4.40E-02	4.30E-02	4.70E-02
G	0.10	4.60E-02	4.60E-02	4.60E-02
H	0.03	4.50E-02	4.50E-02	4.50E-02

Yield (mg/mL): 0.112 Dilution Factor: 10×

Protein purified on 11/7/2024 and assayed on 11/14/2024.
Data uploaded by emrldcaven and last updated on 2024-12-12 07:40:31.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.85E+00	3.82E+00	3.64E+00
B	25.00	3.85E+00	3.69E+00	3.38E+00
C	8.33	3.51E+00	3.25E+00	2.77E+00
D	2.78	2.55E+00	2.30E+00	1.79E+00
E	0.93	1.37E+00	1.04E+00	6.92E-01
F	0.31	6.51E-01	5.09E-01	3.63E-01
G	0.10	2.55E-01	2.36E-01
H	0.03	4.20E-02	4.20E-02	4.60E-02

Yield (mg/mL): 1.286 Dilution Factor: 10×

Protein purified on 11/7/2024 and assayed on 11/14/2024.
Data uploaded by Autumnbayerl and last updated on 2024-12-05 09:17:55.

Rosetta Score for I327G: 10.057

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.