Raw Data for BglB Variant W264Q

Expression Data

SDS-PAGE
 0.453

Kinetic Assay Data

slope of W264Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 94.745 106.188 121.653
B 25.00 97.94 98.09 105.63
C 8.33 73.437 72.173 76.68
D 2.78 37.332 38.997 48.104
E 0.93 17.18 18.557 19.763
F 0.31 6.584 5.574 7.71
G 0.10 2.526 2.376 2.347
H 0.03 0.11 0.089 0.05
 0.453      10×

Protein purified on 11/7/2024 and assayed on 11/14/2024.
Data uploaded by Smithmg24 and last updated on 2024-12-20 20:42:26.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 356.368 351.631 393.404
B 25.00 14.043 14.79
C 8.33
D 2.78
E 0.93
F 0.31
G 0.10 7.053 5.824 5.537
H 0.03 0 0
 0.449      10×

Protein purified on 11/7/24 and assayed on 11/14/24.
Data uploaded by KylerThruston and last updated on 2024-12-05 12:06:35.


Thermostability Assay Data

Data missing or deleted from the database.


Reference Data for WT

slope of W264Q A420 nm (min−1)
T (°C) 1 2 3
A 30.0
B 48.3 0.6 0.5
C 45.7 1.194 1.165 1.34
D 42.4
E 37.7 227.13 217.43 226.02
F 33.6 267.52 316.21
G 31.3 181.85 234.03 226.79
H 50.0 231.05 218.11

Protein purified on 11/7/24 and assayed on 11/21/24.
Data uploaded by KylerThruston and last updated on 2024-12-05 12:16:07.


Design Data

4.861

Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.