Raw Data for BglB Variant V314L

Expression Data

SDS-PAGE
 7.152

Kinetic Assay Data

slope of V314L A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.51E-01 3.57E-01 3.68E-01
B 25.00 3.26E-01 3.21E-01 3.26E-01
C 8.33 2.42E-01 2.51E-01 2.50E-01
D 2.78 2.53E-01 2.48E-01 2.40E-01
E 0.93 1.59E-01 1.73E-01 1.60E-01
F 0.31 8.40E-02 9.17E-02 1.06E-01
G 0.10 3.42E-02 3.75E-02 4.14E-02
H 0.03
 7.152      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Lizbethxmarie and last updated on 2024-11-22 08:17:58.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.31E-01 1.35E-01 1.35E-01
B 25.00 1.20E-01 1.16E-01 1.22E-01
C 8.33
D 2.78 6.60E-02 6.30E-02 5.76E-02
E 0.93 3.20E-02 2.96E-02 2.58E-02
F 0.31 1.46E-02 1.38E-02 1.10E-02
G 0.10 6.00E-03 6.15E-03 6.30E-03
H 0.03 0 0
 5.065      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:31:50.


Thermostability Assay Data

slope of V314L A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 4.50E-04 9.00E-04
B 48.3 1.20E-03 9.00E-04 6.00E-04
C 45.7 1.05E-03 1.05E-03 1.05E-03
D 42.4 2.46E-02 2.01E-02 1.88E-02
E 37.7 1.16E+00 1.22E+00 1.12E+00
F 33.6 1.59E+00 1.48E+00 1.60E+00
G 31.3 1.71E+00 1.44E+00 1.57E+00
H 30.0 1.63E+00 1.74E+00 1.78E+00

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Lizbethxmarie and last updated on 2024-11-22 08:20:20.


Reference Data for WT

slope of V314L A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 7.50E-04
C 45.7 7.50E-04 9.00E-04 9.00E-04
D 42.4 1.56E-02 1.34E-02 1.02E-02
E 37.7 1.30E-01 1.19E-01 9.75E-02
F 33.6 1.25E-01 1.17E-01 1.05E-01
G 31.3 1.40E-01 1.25E-01 1.12E-01
H 30.0 1.32E-01 1.20E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:40:57.


Design Data

2.324

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.