Raw Data for BglB Variant V314D

Expression Data

SDS-PAGE
 8.319

Kinetic Assay Data

slope of V314D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.06E-01 3.27E-01 3.11E-01
B 25.00 2.42E-01 2.23E-01 2.20E-01
C 8.33 1.79E-01 2.01E-01 1.90E-01
D 2.78 1.18E-01 1.19E-01 1.22E-01
E 0.93 4.92E-02 5.42E-02 6.42E-02
F 0.31 1.67E-02 2.01E-02 1.98E-02
G 0.10 6.30E-03 6.60E-03 8.25E-03
H 0.03 6.00E-04 4.50E-04
 8.319      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by jbent021 and last updated on 2024-11-22 08:20:33.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.69E-01 3.76E-01 3.61E-01
B 25.00 2.82E-01 3.06E-01 2.98E-01
C 8.33 2.14E-01 2.26E-01 2.24E-01
D 2.78 1.21E-01 1.31E-01 1.34E-01
E 0.93 5.94E-02 4.91E-02 5.97E-02
F 0.31 1.70E-02 1.71E-02 1.80E-02
G 0.10 6.00E-03 6.45E-03 7.05E-03
H 0.03
 6.75      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 08:39:26.


Thermostability Assay Data

slope of V314D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 6.00E-04
C 45.7 6.00E-04 6.00E-04
D 42.4 1.50E-03 1.50E-03
E 37.7 9.36E-02 9.95E-02
F 33.6 1.73E-01 1.57E-01 1.63E-01
G 31.3 1.45E-01 1.48E-01
H 30.0 1.66E-01 1.55E-01 1.54E-01

Protein purified on and assayed on .
Data uploaded by jbent021 and last updated on 2024-11-22 08:36:34.


Reference Data for WT

slope of V314D A420 nm (min−1)
T (°C) 1 2 3
A 50.0
B 48.3
C 45.7 7.50E-04 9.00E-04
D 42.4 1.71E-02 1.47E-02 1.38E-02
E 37.7 1.35E-01 1.37E-01 1.33E-01
F 33.6 1.42E-01 1.52E-01 1.56E-01
G 31.3 1.59E-01 1.53E-01 1.55E-01
H 30.0 1.69E-01 1.60E-01 1.66E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 08:39:48.


Design Data

6.655

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.