Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.065

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.31E-01	1.35E-01	1.35E-01
	B	25.00	1.20E-01	1.16E-01	1.22E-01
	C	8.33
	D	2.78	6.60E-02	6.30E-02	5.76E-02
	E	0.93	3.20E-02	2.96E-02	2.58E-02
	F	0.31	1.46E-02	1.38E-02	1.10E-02
	G	0.10	6.00E-03	6.15E-03	6.30E-03
	H	0.03	0	0

Yield (mg/mL): 5.065     Dilution Factor:  100×

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:31:50.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0	0
	B	48.3	6.00E-04	7.50E-04
	C	45.7	7.50E-04	9.00E-04	9.00E-04
	D	42.4	1.56E-02	1.34E-02	1.02E-02
	E	37.7	1.30E-01	1.19E-01	9.75E-02
	F	33.6	1.25E-01	1.17E-01	1.05E-01
	G	31.3	1.40E-01	1.25E-01	1.12E-01
	H	30.0	1.32E-01	1.20E-01

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:40:57.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.