Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 6.238

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.67E-01	4.80E-01	4.65E-01
	B	25.00	3.78E-01	3.95E-01	4.04E-01
	C	8.33	2.65E-01	2.82E-01	2.77E-01
	D	2.78	2.10E-01	1.76E-01	1.89E-01
	E	0.93	9.03E-02	7.88E-02	8.09E-02
	F	0.31	3.33E-02	3.41E-02	3.59E-02
	G	0.10	1.20E-02	1.04E-02	1.04E-02
	H	0.03

Yield (mg/mL): 6.238     Dilution Factor:  100×

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Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 08:40:04.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0	0
	B	48.3	0	0	0
	C	45.7	7.50E-04	7.50E-04	7.50E-04
	D	42.4		1.76E-02	1.43E-02
	E	37.7		2.12E-01	2.16E-01
	F	33.6	2.11E-01	2.19E-01	2.09E-01
	G	31.3	2.10E-01	2.14E-01	2.14E-01
	H	30.0	2.07E-01	2.11E-01	2.10E-01

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 09:32:51.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.