Raw Data for BglB Variant V314A

Expression Data

SDS-PAGE
 2.85

Kinetic Assay Data

slope of V314A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.53E-01 1.52E-01 1.57E-01
B 25.00 1.27E-01 1.26E-01 1.35E-01
C 8.33 1.26E-01 1.07E-01 1.28E-01
D 2.78 7.56E-02 8.87E-02 9.81E-02
E 0.93 3.30E-02 3.27E-02 2.94E-02
F 0.31 1.25E-02 1.37E-02 1.19E-02
G 0.10 3.90E-03 3.90E-03
H 0.03 6.00E-04 7.50E-04 4.50E-04
 2.85      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by jonathanmansoor and last updated on 2024-11-22 08:44:32.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.31E-01 1.35E-01 1.35E-01
B 25.00 1.20E-01 1.16E-01 1.22E-01
C 8.33
D 2.78 6.60E-02 6.30E-02 5.76E-02
E 0.93 3.20E-02 2.96E-02 2.58E-02
F 0.31 1.46E-02 1.38E-02 1.10E-02
G 0.10 6.00E-03 6.15E-03 6.30E-03
H 0.03 0 0
 5.065      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:31:50.


Thermostability Assay Data

slope of V314A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 6.00E-04
B 48.3 0 0
C 45.7 6.00E-04 6.00E-04
D 42.4 2.10E-03 2.10E-03 1.95E-03
E 37.7 4.38E-02 3.98E-02 3.92E-02
F 33.6 4.71E-02 5.04E-02 4.17E-02
G 31.3 6.57E-02 6.06E-02 5.66E-02
H 30.0 6.53E-02 5.45E-02 5.72E-02

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by jonathanmansoor and last updated on 2024-11-22 08:45:19.


Reference Data for WT

slope of V314A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 7.50E-04
C 45.7 7.50E-04 9.00E-04 9.00E-04
D 42.4 1.56E-02 1.34E-02 1.02E-02
E 37.7 1.30E-01 1.19E-01 9.75E-02
F 33.6 1.25E-01 1.17E-01 1.05E-01
G 31.3 1.40E-01 1.25E-01 1.12E-01
H 30.0 1.32E-01 1.20E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:40:57.


Design Data

2.839

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.