Raw Data for BglB Variant V314K

Expression Data

SDS-PAGE
 2.936

Kinetic Assay Data

slope of V314K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.24E-01 2.28E-01
B 25.00 1.91E-01 1.77E-01
C 8.33 1.51E-01 1.46E-01 1.11E-01
D 2.78 1.08E-01 8.85E-02 5.19E-02
E 0.93 5.37E-02 4.01E-02 1.70E-02
F 0.31 1.70E-02 1.47E-02 9.75E-03
G 0.10 5.85E-03 5.10E-03 3.15E-03
H 0.03 6.00E-04
 2.936      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MariOrtiz and last updated on 2024-11-22 08:47:22.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.67E-01 4.80E-01 4.65E-01
B 25.00 3.78E-01 3.95E-01 4.04E-01
C 8.33 2.65E-01 2.82E-01 2.77E-01
D 2.78 2.10E-01 1.76E-01 1.89E-01
E 0.93 9.03E-02 7.88E-02 8.09E-02
F 0.31 3.33E-02 3.41E-02 3.59E-02
G 0.10 1.20E-02 1.04E-02 1.04E-02
H 0.03
 6.238      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 08:40:04.


Thermostability Assay Data

slope of V314K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.50E-04 6.00E-04
B 48.3 6.00E-04 7.50E-04
C 45.7 7.50E-04
D 42.4 1.35E-03 1.20E-03 1.20E-03
E 37.7 3.18E-02 3.57E-02 3.15E-02
F 33.6 4.95E-02 4.62E-02 4.67E-02
G 31.3 8.60E-02 7.08E-02 6.77E-02
H 30.0 7.28E-02 7.20E-02 7.88E-02

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MariOrtiz and last updated on 2024-11-22 08:48:21.


Reference Data for WT

slope of V314K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0 0
C 45.7 7.50E-04 7.50E-04 7.50E-04
D 42.4 1.76E-02 1.43E-02
E 37.7 2.12E-01 2.16E-01
F 33.6 2.11E-01 2.19E-01 2.09E-01
G 31.3 2.10E-01 2.14E-01 2.14E-01
H 30.0 2.07E-01 2.11E-01 2.10E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 09:32:51.


Design Data

2.366

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.