Raw Data for BglB Variant V314T

Expression Data

SDS-PAGE
 2.239

Kinetic Assay Data

slope of V314T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.10E-01 1.11E-01 1.09E-01
B 25.00 9.35E-02 9.45E-02 9.18E-02
C 8.33 6.96E-02 6.93E-02 6.74E-02
D 2.78 6.18E-02 6.11E-02 4.85E-02
E 0.93 2.22E-02 2.30E-02 2.27E-02
F 0.31 7.95E-03 8.40E-03 8.25E-03
G 0.10 2.70E-03 2.85E-03 2.85E-03
H 0.03 7.50E-04 6.00E-04
 2.239      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dpayan and last updated on 2024-11-22 08:45:50.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.67E-01 4.80E-01 4.65E-01
B 25.00 3.78E-01 3.95E-01 4.04E-01
C 8.33 2.65E-01 2.82E-01 2.77E-01
D 2.78 2.10E-01 1.76E-01 1.89E-01
E 0.93 9.03E-02 7.88E-02 8.09E-02
F 0.31 3.33E-02 3.41E-02 3.59E-02
G 0.10 1.20E-02 1.04E-02 1.04E-02
H 0.03
 6.238      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 08:40:04.


Thermostability Assay Data

slope of V314T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 7.50E-04
C 45.7 6.00E-04 7.50E-04
D 42.4 2.10E-03 2.25E-03 2.10E-03
E 37.7 2.96E-02 2.94E-02 2.85E-02
F 33.6 3.87E-02 3.89E-02 3.56E-02
G 31.3 3.89E-02 3.65E-02
H 30.0 4.67E-02 4.52E-02 4.16E-02

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dpayan and last updated on 2024-11-22 08:52:17.


Reference Data for WT

slope of V314T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0 0
C 45.7 7.50E-04 7.50E-04 7.50E-04
D 42.4 1.76E-02 1.43E-02
E 37.7 2.12E-01 2.16E-01
F 33.6 2.11E-01 2.19E-01 2.09E-01
G 31.3 2.10E-01 2.14E-01 2.14E-01
H 30.0 2.07E-01 2.11E-01 2.10E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 09:32:51.


Design Data

3.33

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.