Raw Data for BglB Variant V314F

Expression Data

SDS-PAGE
 5.002

Kinetic Assay Data

slope of V314F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.87E-01 3.14E-01 3.16E-01
B 25.00 2.51E-01 2.61E-01 2.49E-01
C 8.33 1.94E-01 1.95E-01 1.91E-01
D 2.78 1.29E-01 1.33E-01 1.27E-01
E 0.93 5.31E-02 5.94E-02 5.64E-02
F 0.31 2.27E-02 1.95E-02 2.28E-02
G 0.10 6.75E-03 1.10E-02 8.25E-03
H 0.03
 5.002      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by asaiz and last updated on 2024-11-22 08:43:49.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.31E-01 1.35E-01 1.35E-01
B 25.00 1.20E-01 1.16E-01 1.22E-01
C 8.33
D 2.78 6.60E-02 6.30E-02 5.76E-02
E 0.93 3.20E-02 2.96E-02 2.58E-02
F 0.31 1.46E-02 1.38E-02 1.10E-02
G 0.10 6.00E-03 6.15E-03 6.30E-03
H 0.03 0 0
 5.065      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:31:50.


Thermostability Assay Data

slope of V314F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.50E-03 0 6.00E-04
B 48.3 7.50E-04 4.50E-04
C 45.7 1.05E-03 6.00E-04
D 42.4 1.20E-03 1.05E-03 1.05E-03
E 37.7 3.98E-02 3.96E-02
F 33.6 6.21E-02 7.64E-02 6.65E-02
G 31.3 7.46E-02 6.56E-02
H 30.0 7.29E-02 7.13E-02 7.04E-02

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by asaiz and last updated on 2024-11-22 08:44:41.


Reference Data for WT

slope of V314F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 7.50E-04
C 45.7 7.50E-04 9.00E-04 9.00E-04
D 42.4 1.56E-02 1.34E-02 1.02E-02
E 37.7 1.30E-01 1.19E-01 9.75E-02
F 33.6 1.25E-01 1.17E-01 1.05E-01
G 31.3 1.40E-01 1.25E-01 1.12E-01
H 30.0 1.32E-01 1.20E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by MTill016 and last updated on 2024-11-22 08:40:57.


Design Data

8.16

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.