Raw Data for BglB Variant V314H

Expression Data

SDS-PAGE
 6.951

Kinetic Assay Data

slope of V314H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.73E-01 4.61E-01
B 25.00 4.01E-01 3.99E-01 4.19E-01
C 8.33 2.96E-01 3.37E-01 2.91E-01
D 2.78 2.21E-01 2.54E-01 2.47E-01
E 0.93 9.65E-02 8.61E-02 8.10E-02
F 0.31 3.54E-02 3.05E-02 3.24E-02
G 0.10 1.16E-02 1.41E-02 1.11E-02
H 0.03 6.00E-04 7.50E-04
 6.951      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by AnnieLassegue and last updated on 2024-11-22 08:48:46.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.67E-01 4.80E-01 4.65E-01
B 25.00 3.78E-01 3.95E-01 4.04E-01
C 8.33 2.65E-01 2.82E-01 2.77E-01
D 2.78 2.10E-01 1.76E-01 1.89E-01
E 0.93 9.03E-02 7.88E-02 8.09E-02
F 0.31 3.33E-02 3.41E-02 3.59E-02
G 0.10 1.20E-02 1.04E-02 1.04E-02
H 0.03
 6.238      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 08:40:04.


Thermostability Assay Data

slope of V314H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 4.50E-04 4.50E-04
C 45.7 1.05E-03 6.00E-04
D 42.4 1.65E-03 1.65E-03 1.50E-03
E 37.7 1.07E-01 1.14E-01 1.17E-01
F 33.6 1.76E-01 1.75E-01 1.71E-01
G 31.3 1.93E-01 1.79E-01 1.83E-01
H 30.0 1.91E-01 1.74E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by AnnieLassegue and last updated on 2024-11-22 09:03:37.


Reference Data for WT

slope of V314H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0 0
C 45.7 7.50E-04 7.50E-04 7.50E-04
D 42.4 1.76E-02 1.43E-02
E 37.7 2.12E-01 2.16E-01
F 33.6 2.11E-01 2.19E-01 2.09E-01
G 31.3 2.10E-01 2.14E-01 2.14E-01
H 30.0 2.07E-01 2.11E-01 2.10E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by dalva214 and last updated on 2024-11-22 09:32:51.


Design Data

5.607

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.