Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.62

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.63E-01	3.70E-01	3.60E-01
	B	25.00	3.03E-01	2.99E-01	3.03E-01
	C	8.33	2.61E-01	2.44E-01	2.33E-01
	D	2.78	1.81E-01	1.97E-01	2.19E-01
	E	0.93	9.44E-02	8.88E-02	8.96E-02
	F	0.31	3.45E-02	3.18E-02	3.00E-02
	G	0.10	1.17E-02	1.14E-02	1.23E-02
	H	0.03

Yield (mg/mL): 2.62     Dilution Factor:  100×

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Zariahnasir and last updated on 2024-11-22 08:47:09.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04		6.00E-04
	B	48.3	4.50E-04		4.50E-04
	C	45.7	6.00E-04		6.00E-04
	D	42.4	1.92E-02	1.80E-02	1.88E-02
	E	37.7	2.00E-01	1.92E-01	1.92E-01
	F	33.6	2.14E-01	2.16E-01	2.11E-01
	G	31.3	2.30E-01	2.23E-01	2.09E-01
	H	30.0	2.14E-01	2.12E-01	2.11E-01

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Zariahnasir and last updated on 2024-11-22 08:48:45.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.