D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P251W

Expression Data

Yield (mg/mL): 0.2193

Kinetic Assay Data

**slope of P251W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.65E-03	1.95E-03	2.10E-03
B	25.00	0.00135	1.50E-03	1.50E-03
C	8.33	1.50E-03	1.35E-03	1.35E-03
D	2.78	1.05E-03	1.05E-03	9.00E-04
E	0.93	7.50E-04	7.50E-04	7.50E-04
F	0.31		4.50E-04	6.00E-04
G	0.10	0		0
H	0.03	0	0	0

Yield (mg/mL): 0.2193 Dilution Factor: 10×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by IsabellaBauchiero and last updated on 2024-11-22 13:10:09.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.34E-01	2.40E-01	2.35E-01
B	25.00	1.98E-01	1.97E-01	2.04E-01
C	8.33	2.02E-01	1.77E-01	2.10E-01
D	2.78	1.61E-01	1.64E-01	1.76E-01
E	0.93	6.56E-02	6.08E-02	6.23E-02
F	0.31	2.64E-02	2.54E-02	2.58E-02
G	0.10	1.01E-02	9.00E-03	9.75E-03
H	0.03	0	0	0

Yield (mg/mL): 3.107 Dilution Factor: 100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by IsabellaBauchiero and last updated on 2024-11-22 13:22:31.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of P251W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	0	0	0
C	45.7	9.00E-04	6.00E-04	7.50E-04
D	42.4	1.25E-02	1.17E-02	1.10E-02
E	37.7		1.27E-01	1.28E-01
F	33.6	1.32E-01	1.36E-01	1.37E-01
G	31.3	1.39E-01	1.33E-01	1.37E-01
H	30.0	1.40E-01	1.37E-01	1.31E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Kaysenst and last updated on 2024-11-22 13:27:21.

Design Data

Rosetta Score for P251W: 219.135

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.