Raw Data for BglB Variant P251F

Expression Data

SDS-PAGE
 0.2894

Kinetic Assay Data

slope of P251F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.80E-03 8.10E-03
B 25.00 6.00E-03 6.30E-03 6.60E-03
C 8.33 4.80E-03 5.40E-03 5.10E-03
D 2.78 4.05E-03 4.05E-03 3.90E-03
E 0.93 1.95E-03 2.10E-03 2.10E-03
F 0.31 1.05E-03 9.00E-04 9.00E-04
G 0.10 7.50E-04 7.50E-04 7.50E-04
H 0.03 0 0
 0.2894      10×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by lacyjuli and last updated on 2024-11-22 13:26:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.36E-01 3.75E-01
B 25.00 2.97E-01 2.96E-01 3.02E-01
C 8.33 2.74E-01 2.76E-01 2.77E-01
D 2.78 1.63E-01 1.86E-01 1.64E-01
E 0.93 6.99E-02 7.02E-02 8.04E-02
F 0.31 3.14E-02 3.60E-02 2.96E-02
G 0.10 1.01E-02 1.11E-02 1.35E-02
H 0.03 0 0
 5.215      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by katherineg1230 and last updated on 2024-11-22 13:36:46.


Thermostability Assay Data

slope of P251F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0 0
C 45.7 0 0 0
D 42.4 6.00E-04 6.00E-04 6.00E-04
E 37.7 2.25E-03 1.95E-03 1.80E-03
F 33.6 2.85E-03 2.85E-03 3.00E-03
G 31.3 3.30E-03 2.85E-03 3.15E-03
H 30.0 3.90E-03 3.45E-03 4.05E-03

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by lacyjuli and last updated on 2024-11-22 13:32:12.


Reference Data for WT

slope of P251F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.50E-04
D 42.4 1.65E-02 1.56E-02 1.61E-02
E 37.7 1.91E-01 1.81E-01 1.90E-01
F 33.6 2.26E-01 2.25E-01 2.12E-01
G 31.3 1.83E-01 1.88E-01 1.75E-01
H 30.0 1.94E-01 1.84E-01 1.78E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by katherineg1230 and last updated on 2024-11-22 13:37:23.


Design Data

494.791

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.