Raw Data for BglB Variant P251A

Expression Data

SDS-PAGE
 4.277

Kinetic Assay Data

slope of P251A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.07E-01 2.00E-01
B 25.00
C 8.33 2.13E-01 2.05E-01 2.33E-01
D 2.78 1.17E-01 1.41E-01 1.12E-01
E 0.93 5.84E-02 5.28E-02 5.00E-02
F 0.31 2.09E-02 1.86E-02 1.92E-02
G 0.10 7.20E-03 5.10E-03 6.45E-03
H 0.03 0 0 0
 4.277      100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by dcama032 and last updated on 2024-11-22 13:43:14.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.07E-01 3.02E-01 2.72E-01
B 25.00 2.52E-01 2.48E-01 2.46E-01
C 8.33 2.34E-01 2.43E-01 2.25E-01
D 2.78 1.20E-01 1.32E-01 1.35E-01
E 0.93 5.48E-02 5.48E-02 5.48E-02
F 0.31 2.07E-02 2.12E-02 2.22E-02
G 0.10 7.50E-03 7.05E-03 7.35E-03
H 0.03 0 7.50E-04 4.50E-04
 4.121      100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:08:38.


Thermostability Assay Data

slope of P251A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 6.00E-04
C 45.7 6.00E-04 7.50E-04 7.50E-04
D 42.4 4.50E-03 4.65E-03 4.35E-03
E 37.7 8.70E-02 9.81E-02 9.63E-02
F 33.6 1.38E-01 1.43E-01 1.32E-01
G 31.3 1.21E-01 1.29E-01 1.36E-01
H 30.0 1.58E-01 1.52E-01

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by dcama032 and last updated on 2024-11-22 13:44:54.


Reference Data for WT

slope of P251A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 4.50E-04
B 48.3 6.00E-04 6.00E-04
C 45.7 9.00E-04 9.00E-04 1.05E-03
D 42.4 1.61E-02 1.59E-02
E 37.7 1.27E-01 1.39E-01 1.29E-01
F 33.6 1.67E-01 1.46E-01 1.58E-01
G 31.3 1.74E-01 1.56E-01 1.51E-01
H 30.0 1.51E-01 1.41E-01

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:12:21.


Design Data

-9.616

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.