Raw Data for BglB Variant F289C

Expression Data

Yield (mg/mL): 0.126

Thermostability Assay Data

slope of F289C A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		1.50E-04	1.50E-04
	B	48.3	1.50E-04	2.00E-04
	C	45.7	1.50E-04	2.00E-04	0.00E+00
	D	42.4		2.50E-04	2.50E-04
	E	37.7	2.00E-04	1.50E-04	1.00E-04
	F	33.6		2.00E-04	2.00E-04
	G	31.3	2.00E-04	2.00E-04	2.00E-04
	H	30.0	2.50E-04	2.50E-04	3.00E-04

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Protein purified on 11/14/24 and assayed on 11/16/24.
Data uploaded by nathangreen and last updated on 2024-12-05 12:49:03.

Reference Data for WT

slope of F289C A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.00E-04	2.00E-04	2.00E-04
	B	48.3		0.00E+00	0.00E+00
	C	45.7	2.50E-04	3.00E-04	1.50E-04
	D	42.4	9.00E-04	7.00E-04	8.50E-04
	E	37.7	4.55E-03	3.95E-03	4.15E-03
	F	33.6	4.95E-03	4.55E-03	4.05E-03
	G	31.3	4.65E-03	5.05E-03	4.80E-03
	H	30.0	5.30E-03	5.20E-03	5.40E-03

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Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by morganmcd and last updated on 2024-12-05 12:54:29.

Design Data

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.