Raw Data for BglB Variant F289W
Expression Data
1.542
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 2.62E-02 | 2.46E-02 | 2.35E-02 | ||
| B | 25.00 | 1.05E-02 | 1.01E-02 | 1.02E-02 | ||
| C | 8.33 | 3.80E-03 | 3.55E-03 | 3.80E-03 | ||
| D | 2.78 | 1.60E-03 | 1.70E-03 | 1.75E-03 | ||
| E | 0.93 | 8.50E-04 | 7.50E-04 | 6.50E-04 | ||
| F | 0.31 | 3.00E-04 | 5.00E-04 | 4.50E-04 | ||
| G | 0.10 | 2.50E-04 | 6.00E-04 | |||
| H | 0.03 | 0.00E+00 | 2.50E-04 |
Protein purified on 10/31/24 and assayed on 11/5/24.
Data uploaded by paymaun and last updated on 2024-12-05 12:30:13.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 2.37E-02 | 2.33E-02 | 2.15E-02 | ||
| B | 25.00 | 1.05E-02 | 1.01E-02 | 8.50E-03 | ||
| C | 8.33 | 3.35E-03 | 3.10E-03 | 3.30E-03 | ||
| D | 2.78 | 1.40E-03 | 1.50E-03 | 1.65E-03 | ||
| E | 0.93 | 8.50E-04 | 6.50E-04 | 6.00E-04 | ||
| F | 0.31 | 4.50E-04 | 3.50E-04 | 5.50E-04 | ||
| G | 0.10 | 2.50E-04 | 3.00E-04 | 3.50E-04 | ||
| H | 0.03 | 2.00E-04 | 2.00E-04 | 2.00E-04 |
Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by hananalhamami and last updated on 2024-12-05 12:43:58.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0.00E+00 | 3.50E-04 | 5.50E-04 | ||
| B | 48.3 | 1.00E-04 | 1.50E-04 | 0.00E+00 | ||
| C | 45.7 | 2.50E-04 | 2.00E-04 | 2.00E-04 | ||
| D | 42.4 | 3.50E-04 | 3.50E-04 | 3.50E-04 | ||
| E | 37.7 | 3.95E-03 | 3.70E-03 | 3.80E-03 | ||
| F | 33.6 | 4.25E-03 | 4.60E-03 | 4.45E-03 | ||
| G | 31.3 | 4.80E-03 | 4.50E-03 | 4.85E-03 | ||
| H | 30.0 | 4.90E-03 | 4.95E-03 | 4.80E-03 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 2.50E-04 | 2.50E-04 | |||
| B | 48.3 | 2.50E-04 | 2.00E-04 | |||
| C | 45.7 | 2.50E-04 | 2.00E-04 | |||
| D | 42.4 | 9.00E-04 | 9.00E-04 | 1.05E-03 | ||
| E | 37.7 | 3.50E-03 | 3.30E-03 | 3.70E-03 | ||
| F | 33.6 | 3.80E-03 | 3.70E-03 | 3.85E-03 | ||
| G | 31.3 | 3.80E-03 | 3.90E-03 | |||
| H | 30.0 | 4.15E-03 | 4.25E-03 | 4.30E-03 |
Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by hananalhamami and last updated on 2024-12-05 12:48:37.
Design Data
Citations
The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.