Raw Data for BglB Variant I20C
Expression Data
0.59
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.97E-02 | 2.02E-02 | 2.77E-02 | ||
| B | 25.00 | 1.35E-02 | 1.49E-02 | 1.52E-02 | ||
| C | 8.33 | 8.10E-03 | 9.90E-03 | 9.30E-03 | ||
| D | 2.78 | 4.25E-03 | 4.40E-03 | 4.10E-03 | ||
| E | 0.93 | 1.95E-03 | 2.20E-03 | 2.00E-03 | ||
| F | 0.31 | 8.50E-04 | 8.50E-04 | 6.50E-04 | ||
| G | 0.10 | 4.00E-04 | 4.00E-04 | 4.00E-04 | ||
| H | 0.03 | 0.00E+00 | 5.00E-04 | 2.00E-04 |
Protein purified on 10/30/24 and assayed on 11/2/24.
Data uploaded by Reilly-Bowen and last updated on 2024-12-04 11:44:14.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.37E-01 | 1.62E-01 | 1.57E-01 | ||
| B | 25.00 | 1.29E-01 | 1.30E-01 | 1.40E-01 | ||
| C | 8.33 | 1.17E-01 | 1.47E-01 | 1.29E-01 | ||
| D | 2.78 | 7.69E-02 | 7.98E-02 | |||
| E | 0.93 | 3.00E-02 | 3.08E-02 | |||
| F | 0.31 | 7.95E-03 | 1.27E-02 | 1.09E-02 | ||
| G | 0.10 | 3.75E-03 | 4.05E-03 | |||
| H | 0.03 | 1.50E-04 | 1.50E-04 |
Protein purified on 10/30/2024 and assayed on 11/4/2024.
Data uploaded by pattycake and last updated on 2024-12-04 11:51:30.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 3.00E-04 | 2.50E-04 | |||
| B | 48.3 | 0.00E+00 | 0.00E+00 | 0.00E+00 | ||
| C | 45.7 | 0.00E+00 | 0.00E+00 | 0.00E+00 | ||
| D | 42.4 | 3.50E-04 | 4.00E-04 | |||
| E | 37.7 | 7.50E-04 | 8.00E-04 | 9.50E-04 | ||
| F | 33.6 | 1.00E-03 | 9.50E-04 | 1.05E-03 | ||
| G | 31.3 | 9.50E-04 | 9.00E-04 | 9.50E-04 | ||
| H | 30.0 | 8.50E-04 | 1.25E-03 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0.00E+00 | 0.00E+00 | |||
| B | 48.3 | 0.00E+00 | 0.00E+00 | |||
| C | 45.7 | 0.00E+00 | 0.00E+00 | 0.00E+00 | ||
| D | 42.4 | 7.00E-04 | 6.00E-04 | 8.00E-04 | ||
| E | 37.7 | 2.65E-03 | 2.45E-03 | 2.75E-03 | ||
| F | 33.6 | 2.50E-03 | 2.65E-03 | 2.75E-03 | ||
| G | 31.3 | 2.75E-03 | 2.70E-03 | 2.80E-03 | ||
| H | 30.0 | 2.20E-03 | 2.80E-03 | 2.75E-03 |
Protein purified on 10/30/2024 and assayed on 11/9/2024.
Data uploaded by rhodosev and last updated on 2024-12-04 12:01:23.
Design Data
Citations
The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.