Raw Data for BglB Variant D260E
Expression Data
0.23
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.21E-02 | 1.15E-02 | 1.19E-02 | ||
| B | 25.00 | 8.65E-03 | 8.20E-03 | 8.50E-03 | ||
| C | 8.33 | 6.90E-03 | 6.40E-03 | 7.10E-03 | ||
| D | 2.78 | 4.90E-03 | 4.75E-03 | 4.30E-03 | ||
| E | 0.93 | 2.30E-03 | 2.10E-03 | 2.10E-03 | ||
| F | 0.31 | 1.30E-03 | 1.00E-03 | 9.00E-04 | ||
| G | 0.10 | 5.50E-04 | 5.00E-04 | 5.00E-04 | ||
| H | 0.03 | 0.00E+00 | 0.00E+00 |
Protein purified on 11/4/2024 and assayed on 11/9/2024.
Data uploaded by kimj1282 and last updated on 2024-12-04 11:54:10.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 2.22E-01 | 2.15E-01 | 2.16E-01 | ||
| B | 25.00 | 1.76E-01 | 1.74E-01 | 1.69E-01 | ||
| C | 8.33 | 1.35E-01 | 1.36E-01 | 1.40E-01 | ||
| D | 2.78 | 8.66E-02 | 8.86E-02 | 8.99E-02 | ||
| E | 0.93 | 4.48E-02 | 4.22E-02 | 4.84E-02 | ||
| F | 0.31 | 1.72E-02 | 1.68E-02 | 1.70E-02 | ||
| G | 0.10 | 6.05E-03 | 5.50E-03 | 6.60E-03 | ||
| H | 0.03 | 0.00E+00 | 1.50E-04 | 2.00E-04 |
Protein purified on 11/4/2024 and assayed on 11/9/2024.
Data uploaded by kimj1282 and last updated on 2024-12-04 11:57:44.
Thermostability Assay Data
Data missing or deleted from the database.
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0.00E+00 | 0.00E+00 | |||
| B | 48.3 | 2.00E-04 | 2.00E-04 | |||
| C | 45.7 | 1.50E-04 | 3.50E-04 | |||
| D | 42.4 | 8.50E-04 | 9.50E-04 | |||
| E | 37.7 | 4.35E-03 | 4.75E-03 | 3.15E-03 | ||
| F | 33.6 | 4.35E-03 | 4.75E-03 | 4.10E-03 | ||
| G | 31.3 | 4.25E-03 | 4.75E-03 | 4.35E-03 | ||
| H | 30.0 | 4.45E-03 | 4.75E-03 | 4.45E-03 |
Protein purified on 10/30/2024 and assayed on 11/9/2024.
Data uploaded by Tanner and last updated on 2024-12-04 11:59:59.
Design Data
Citations
The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.