Raw Data for BglB Variant N404S

Expression Data

SDS-PAGE
 0.764

Kinetic Assay Data

slope of N404S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.07500 0.07400 0.07900
B 25.00 0.05150 0.04850 0.04550
C 8.33 0.02300 0.02300 0.02300
D 2.78 0.00900 0.00900 0.00900
E 0.93 0.00300 0.00300 0.00350
F 0.31 0.00100 0.00100
G 0.10 0.00050 0.00050
H 0.03 0.00000 0.00000
 0.764      10×

Protein purified on 11/5/23 and assayed on 11/12/23.
Data uploaded by JustinMao and last updated on 2024-12-03 14:22:26.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of N404S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04
B 48.3 5.00E-04 5.00E-04
C 45.7 2.50E-03 2.50E-03 3.00E-03
D 42.4 1.05E-02 1.10E-02 1.00E-02
E 37.7 1.15E-02 1.15E-02 1.10E-02
F 33.6 1.20E-02 1.15E-02 1.05E-02
G 31.3 1.05E-02 1.15E-02 1.15E-02
H 30.0 1.15E-02 1.20E-02 1.15E-02

Protein purified on 11/5/24 and assayed on 11/19/24.
Data uploaded by JustinMao and last updated on 2024-12-03 14:24:38.


Reference Data for WT

slope of N404S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.