Raw Data for BglB Variant N220R

Expression Data

SDS-PAGE
 0.59

Kinetic Assay Data

slope of N220R A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 6.90E-02 7.10E-02 7.10E-02
B 25.00 5.55E-02 5.60E-02 5.45E-02
C 8.33 4.15E-02 4.10E-02 3.90E-02
D 2.78 2.35E-02 2.50E-02 2.40E-02
E 0.93 1.15E-02 1.10E-02 1.10E-02
F 0.31 4.50E-03 4.50E-03 4.50E-03
G 0.10 1.50E-03 1.50E-03 1.50E-03
H 0.03 5.00E-04 5.00E-04
 0.59      100×

Protein purified on 10/22/24 and assayed on 11/3/24.
Data uploaded by susannahtuder and last updated on 2024-12-03 14:01:27.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of N220R A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04
B 48.3 5.00E-04 5.00E-04
C 45.7 5.00E-04 5.00E-04
D 42.4 1.30E-02 1.30E-02 1.10E-02
E 37.7 3.35E-02 3.20E-02 3.00E-02
F 33.6 3.90E-02 3.85E-02 3.55E-02
G 31.3 4.30E-02 4.10E-02 3.80E-02
H 30.0 4.50E-02 4.35E-02 3.90E-02

Protein purified on 10/22/24 and assayed on 11/12/24.
Data uploaded by susannahtuder and last updated on 2024-12-03 14:12:11.


Reference Data for WT

slope of N220R A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Design Data

-4.692

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.