Raw Data for BglB Variant Y375T
Expression Data
0.431
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 7.50E-03 | 8.50E-03 | 7.50E-03 | ||
| B | 25.00 | 3.00E-03 | 3.50E-03 | 3.50E-03 | ||
| C | 8.33 | 2.00E-03 | 1.50E-03 | 2.00E-03 | ||
| D | 2.78 | 1.00E-03 | 1.00E-03 | 1.00E-03 | ||
| E | 0.93 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| F | 0.31 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| G | 0.10 | 5.00E-04 | 5.00E-04 | |||
| H | 0.03 |
Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by GraceD and last updated on 2024-12-04 14:01:38.
Thermostability Assay Data
Data missing or deleted from the database.
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0 | 7.00E-04 | |||
| B | 48.3 | 4.00E-04 | 5.00E-04 | 6.00E-04 | ||
| C | 45.7 | 6.00E-04 | 9.00E-04 | 7.00E-04 | ||
| D | 42.4 | 4.12E-02 | 3.96E-02 | 4.71E-02 | ||
| E | 37.7 | 2.14E-01 | 1.91E-01 | 2.10E-01 | ||
| F | 33.6 | 2.47E-01 | 2.35E-01 | 2.36E-01 | ||
| G | 31.3 | 2.18E-01 | 2.14E-01 | 2.05E-01 | ||
| H | 30.0 | 2.62E-01 | 2.56E-01 | 2.62E-01 |
Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.
Design Data
Citations
The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.