Raw Data for BglB Variant C167T
Expression Data
0.203
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 2.07E-01 | 2.29E-01 | |||
| B | 25.00 | 1.77E-01 | 1.91E-01 | 1.89E-01 | ||
| C | 8.33 | 1.48E-01 | 1.58E-01 | 1.72E-01 | ||
| D | 2.78 | 1.10E-01 | 1.18E-01 | 1.16E-01 | ||
| E | 0.93 | 6.55E-02 | 6.75E-02 | 8.55E-02 | ||
| F | 0.31 | 3.00E-02 | 2.95E-02 | 3.30E-02 | ||
| G | 0.10 | 1.15E-02 | 1.10E-02 | 1.30E-02 | ||
| H | 0.03 | 5.00E-04 | 5.00E-04 |
Protein purified on 23/10/2024 and assayed on 06/11/2024.
Data uploaded by RoryB and last updated on 2024-12-04 13:51:23.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.88E-01 | 1.82E-01 | 1.79E-01 | ||
| B | 25.00 | 1.49E-01 | 1.52E-01 | 1.47E-01 | ||
| C | 8.33 | 1.35E-01 | 1.37E-01 | 1.39E-01 | ||
| D | 2.78 | 6.51E-02 | 6.22E-02 | 6.55E-02 | ||
| E | 0.93 | 4.34E-02 | 4.43E-02 | 4.18E-02 | ||
| F | 0.31 | 2.02E-02 | 1.64E-02 | 1.69E-02 | ||
| G | 0.10 | 9.45E-03 | 1.03E-02 | 7.65E-03 | ||
| H | 0.03 | 3.40E-03 | 3.45E-03 | 2.45E-03 |
Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | |||||
| B | 48.3 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| C | 45.7 | 1.00E-03 | 1.00E-03 | 1.00E-03 | ||
| D | 42.4 | 1.50E-03 | 1.50E-03 | |||
| E | 37.7 | 5.50E-03 | 5.50E-03 | 5.50E-03 | ||
| F | 33.6 | 8.50E-03 | 7.50E-03 | 7.50E-03 | ||
| G | 31.3 | 9.50E-03 | 9.50E-03 | 9.50E-03 | ||
| H | 30.0 | 1.15E-02 | 1.10E-02 | 1.20E-02 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0 | 7.00E-04 | |||
| B | 48.3 | 4.00E-04 | 5.00E-04 | 6.00E-04 | ||
| C | 45.7 | 6.00E-04 | 9.00E-04 | 7.00E-04 | ||
| D | 42.4 | 4.12E-02 | 3.96E-02 | 4.71E-02 | ||
| E | 37.7 | 2.14E-01 | 1.91E-01 | 2.10E-01 | ||
| F | 33.6 | 2.47E-01 | 2.35E-01 | 2.36E-01 | ||
| G | 31.3 | 2.18E-01 | 2.14E-01 | 2.05E-01 | ||
| H | 30.0 | 2.62E-01 | 2.56E-01 | 2.62E-01 |
Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.
Design Data
Citations
The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.