D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D128E

Yield (mg/mL): 0.2

**slope of D128E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.00E-02	3.05E-02	2.85E-02
B	25.00	2.30E-02	2.40E-02	2.25E-02
C	8.33	1.25E-02	1.60E-02	1.60E-02
D	2.78	1.10E-02		1.00E-02
E	0.93	5.00E-03	4.50E-03	5.00E-03
F	0.31	2.00E-03	2.00E-03	2.00E-03
G	0.10	3.00E-03	1.50E-03	1.00E-03
H	0.03	0.00E+00	1.48E-16

Yield (mg/mL): 0.2 Dilution Factor: 100×

Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by CalebHwang and last updated on 2024-12-04 14:04:31.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.88E-01	1.82E-01	1.79E-01
B	25.00	1.49E-01	1.52E-01	1.47E-01
C	8.33	1.35E-01	1.37E-01	1.39E-01
D	2.78	6.51E-02	6.22E-02	6.55E-02
E	0.93	4.34E-02	4.43E-02	4.18E-02
F	0.31	2.02E-02	1.64E-02	1.69E-02
G	0.10	9.45E-03	1.03E-02	7.65E-03
H	0.03	3.40E-03	3.45E-03	2.45E-03

Yield (mg/mL): 2.377 Dilution Factor: 100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.

**slope of D128E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.00E+00		0.00E+00
B	48.3	5.00E-04		5.00E-04
C	45.7	1.00E-03		1.00E-03
D	42.4	1.30E-02	1.25E-02	1.00E-02
E	37.7	4.10E-02	4.25E-02	3.95E-02
F	33.6	5.65E-02	5.90E-02	5.30E-02
G	31.3	5.95E-02	6.05E-02	5.55E-02
H	30.0	6.15E-02	5.75E-02	5.50E-02

Protein purified on 10/23/24 and assayed on 11/13/24.
Data uploaded by CalebHwang and last updated on 2024-12-04 14:12:03.

**slope of D128E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	7.00E-04
B	48.3	4.00E-04	5.00E-04	6.00E-04
C	45.7	6.00E-04	9.00E-04	7.00E-04
D	42.4	4.12E-02	3.96E-02	4.71E-02
E	37.7	2.14E-01	1.91E-01	2.10E-01
F	33.6	2.47E-01	2.35E-01	2.36E-01
G	31.3	2.18E-01	2.14E-01	2.05E-01
H	30.0	2.62E-01	2.56E-01	2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.

Rosetta Score for D128E: 1.787

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.