Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.954

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.74E-02	2.73E-02	2.67E-02
	B	25.00	9.65E-03	1.08E-02	1.02E-02
	C	8.33	5.75E-03	4.35E-03	5.40E-03
	D	2.78	1.65E-03	1.60E-03	1.90E-03
	E	0.93	7.50E-04	6.50E-04	8.00E-04
	F	0.31	5.00E-04		4.50E-04
	G	0.10	3.00E-04	3.50E-04	2.50E-04
	H	0.03	0.00E+00	3.50E-04	1.50E-04

Yield (mg/mL): 0.954     Dilution Factor:  100×

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Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by morganmcd and last updated on 2024-12-05 12:51:09.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.00E-04	2.00E-04	2.00E-04
	B	48.3		0.00E+00	0.00E+00
	C	45.7	2.50E-04	3.00E-04	1.50E-04
	D	42.4	9.00E-04	7.00E-04	8.50E-04
	E	37.7	4.55E-03	3.95E-03	4.15E-03
	F	33.6	4.95E-03	4.55E-03	4.05E-03
	G	31.3	4.65E-03	5.05E-03	4.80E-03
	H	30.0	5.30E-03	5.20E-03	5.40E-03

Download these data

Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by morganmcd and last updated on 2024-12-05 12:54:29.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.