Raw Data for BglB Variant E388H

Expression Data

SDS-PAGE
 0.756

Kinetic Assay Data

slope of E388H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78
E 0.93
F 0.31 1.19E-01 1.33E-01 1.40E-01
G 0.10 3.80E-02 4.05E-02 4.40E-02
H 0.03 7.40E-17 0.00E+00
 0.756      10×

Protein purified on 10/24/24 and assayed on 10/31/24.
Data uploaded by RenaudLefevre and last updated on 2024-12-05 15:03:00.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of E388H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04
B 48.4 5.00E-04 5.00E-04 5.00E-04
C 45.9 5.00E-04 5.00E-04 5.00E-04
D 42.2 2.55E-02 2.90E-02 3.60E-02
E 37.4 2.14E-01
F 33.8
G 31.3 2.33E-01
H 30.0 2.49E-01 2.60E-01

Protein purified on 10/24/24 and assayed on 10/31/24.
Data uploaded by RenaudLefevre and last updated on 2024-12-05 15:33:16.


Reference Data for WT

slope of E388H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Design Data

-3.273

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.