Raw Data for BglB Variant M323S

Expression Data

SDS-PAGE
 0.175

Kinetic Assay Data

slope of M323S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.023 0.0235 0.0245
B 25.00 0.017 0.016 0.014
C 8.33 0.009 0.011 0.01
D 2.78 0.005 0.0055 0.0055
E 0.93 0.0025 0.003 0.0025
F 0.31 0.001 0.001
G 0.10 0.001 0.001
H 0.03 0.0005 0.0005 0.0005
 0.175      100×

Protein purified on 10/24/24 and assayed on 11/7/24.
Data uploaded by miagawith and last updated on 2024-12-05 14:44:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of M323S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0025 0.0025 0.003
B 48.3 0.0005 0.0005
C 45.7
D 42.4 0.0015 0.0015 0.0015
E 37.7 0.0085 0.008 0.0075
F 33.6 0.011 0.011 0.0105
G 31.3 0.013 0.0125 0.012
H 30.0 0.013 0.0125 0.0125

Protein purified on 10/24/24 and assayed on 11/14/24.
Data uploaded by miagawith and last updated on 2024-12-05 14:50:41.


Reference Data for WT

slope of M323S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Design Data

5.235

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.