Raw Data for BglB Variant E388H

Expression Data

Yield (mg/mL): 0.7401

Kinetic Assay Data

slope of E388H A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		2.39E-01
	B	25.00	0.199	0.1965	0.192
	C	8.33	0.149	0.1505	0.1425
	D	2.78	0.0915	0.0935	0.085
	E	0.93	0.044	0.0445	0.04
	F	0.31	0.0175	0.0175	0.016
	G	0.10	0.006	0.006	0.0055
	H	0.03	7.40E-17	0	1.48E-16

Yield (mg/mL): 0.7401     Dilution Factor:  10×

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Protein purified on 10/31/24 and assayed on 11/7/24.
Data uploaded by Gettie and last updated on 2024-12-05 15:16:37.

Thermostability Assay Data

slope of E388H A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0005		0.0005
	B	48.3		0.0005	0.0005
	C	45.7	0.0005		0.0005
	D	42.4	0.0165	0.02	0.02
	E	37.7	0.149	0.1515	0.1555
	F	33.6	0.163	0.1665	0.1905
	G	31.3	0.1695	0.1715	0.1745
	H	30.0	0.173	0.17	0.177

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Protein purified on 10/31/24 and assayed on 11/14/24.
Data uploaded by Gettie and last updated on 2024-12-05 15:23:18.

Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.