D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E177W

Yield (mg/mL): 0.198

**slope of E177W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.30E-02	2.35E-02	2.45E-02
B	25.00	1.70E-02	1.60E-02	1.40E-02
C	8.33	9.00E-03	1.10E-02	1.00E-02
D	2.78	5.00E-03	5.50E-03	5.50E-03
E	0.93	2.50E-03	3.00E-03	2.50E-03
F	0.31	1.00E-03		1.00E-03
G	0.10	1.00E-03	1.00E-03
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 0.445 Dilution Factor: 10×

Protein purified on 10/24/2024 and assayed on 11/7/2024.
Data uploaded by GK and last updated on 2024-12-05 15:02:52.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.88E-01	1.82E-01	1.79E-01
B	25.00	1.49E-01	1.52E-01	1.47E-01
C	8.33	1.35E-01	1.37E-01	1.39E-01
D	2.78	6.51E-02	6.22E-02	6.55E-02
E	0.93	4.34E-02	4.43E-02	4.18E-02
F	0.31	2.02E-02	1.64E-02	1.69E-02
G	0.10	9.45E-03	1.03E-02	7.65E-03
H	0.03	3.40E-03	3.45E-03	2.45E-03

Yield (mg/mL): 2.377 Dilution Factor: 100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.

**slope of E177W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	2.50E-03	2.50E-03	3.00E-03
B	48.3		5.00E-04	5.00E-04
C	45.7	0.00E+00	7.40E-17
D	42.4	1.50E-03	1.50E-03	1.50E-03
E	37.7	8.50E-03	8.00E-03	7.50E-03
F	33.6	1.10E-02	1.10E-02	1.05E-02
G	31.3	1.30E-02	1.25E-02	1.20E-02
H	30.0	1.30E-02	1.25E-02	1.25E-02

Protein purified on 10/24/2024 and assayed on 11/14/2024.
Data uploaded by GK and last updated on 2024-12-05 15:15:36.

**slope of E177W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	7.00E-04
B	48.3	4.00E-04	5.00E-04	6.00E-04
C	45.7	6.00E-04	9.00E-04	7.00E-04
D	42.4	4.12E-02	3.96E-02	4.71E-02
E	37.7	2.14E-01	1.91E-01	2.10E-01
F	33.6	2.47E-01	2.35E-01	2.36E-01
G	31.3	2.18E-01	2.14E-01	2.05E-01
H	30.0	2.62E-01	2.56E-01	2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.

Rosetta Score for E177W: 5.157

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.