D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D273W

Yield (mg/mL): 0.991

**slope of D273W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.02E+00	1.12E+00	1.33E+00
B	25.00	8.86E-01	9.09E-01	9.66E-01
C	8.33	7.29E-01	7.86E-01	8.11E-01
D	2.78	5.21E-01	5.44E-01	5.41E-01
E	0.93	3.17E-01	3.66E-01	3.39E-01
F	0.31	1.52E-01	1.90E-01	1.84E-01
G	0.10	9.10E-02	1.14E-01	9.30E-02
H	0.03	4.60E-02	4.40E-02	4.40E-02

Yield (mg/mL): 0.991 Dilution Factor: 10×

Protein purified on 11/14/2024 and assayed on 11/7/2024.
Data uploaded by Daviskh24 and last updated on 2024-12-09 15:19:02.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.40E+00	3.86E+00	3.85E+00
B	25.00	3.76E+00	3.82E+00	3.74E+00
C	8.33	3.60E+00	3.17E+00	3.19E+00
D	2.78	1.89E+00	2.65E+00	2.58E+00
E	0.93	1.10E+00		1.38E+00
F	0.31	5.27E-01	4.76E-01	5.02E-01
G	0.10	2.36E-01	2.08E-01	2.29E-01
H	0.03	4.40E-02	4.40E-02	4.40E-02

Yield (mg/mL): 1.711 Dilution Factor: 10×

Protein purified on 11/14/2024 and assayed on 11/7/2024.
Data uploaded by Daviskh24 and last updated on 2024-12-09 15:24:34.

Rosetta Score for D273W: 8.006

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.