Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.747

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1451	0.1468	0.1443
	B	25.00	0.1451	0.1265	0.1272
	C	8.33	0.1123	0.1155	0.117
	D	2.78	0.1008	0.09	0.1008
	E	0.93	0.04785	0.0432	0.045
	F	0.31	0.0198	0.02085	0.0219
	G	0.10	0.00825	0.00825	0.0087
	H	0.03	0	0	0

Yield (mg/mL): 3.747     Dilution Factor:  100×

Download these data

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 21:54:20.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	0.072	0.08295	0.0729
	B	31.3	0.0852	0.0858	0.07845
	C	33.6	0.07605	0.0759	0.08475
	D	37.7	0.09195	0.0783	0.07785
	E	42.4	0.0099	0.01005	0.0102
	F	45.7	0.0006	0.00075	0.0006
	G	48.3	0.0006	0.0006
	H	50.0	0.00045		0.0006

Download these data

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 22:26:16.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.