Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.747

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1617	0.1625	0.158
	B	25.00	0.1239	0.1195	0.109
	C	8.33	0.1262	0.1027	0.1074
	D	2.78	0.09285	0.0939	0.1031
	E	0.93	0.06105	0.04965	0.0525
	F	0.31	0.02865	0.0297	0.02895
	G	0.10	0.01335	0.0126	0.0117
	H	0.03	0.0006	0.00075

Yield (mg/mL): 3.747     Dilution Factor:  100×

Download these data

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 22:43:22.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	0.1015	0.103	0.09915
	B	31.3	0.09825	0.1021	0.0963
	C	33.6	0.07785	0.08925	0.0915
	D	37.7	0.09075	0.0897	0.087
	E	42.4	0.01305	0.0123	0.012
	F	45.7	0.00075	0.00075	0.0009
	G	48.3	0.00075	0.0006	0.0006002
	H	50.0	0		0

Download these data

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 22:46:32.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.